Generation of nonhydrolyzable ubiquitin–histone mimics
نویسندگان
چکیده
منابع مشابه
nity Chromatography without Nonhydrolyzable GTP Analogues
Rab5, one of the small GTP-binding proteins, is a key regulator both in endocytosis and following membrane-traffi cking events, such as clathrinmediated endocytosis (Christoforidis and Zerial, 2000, 2001; Zhu et al., 2007). Many Rab5-binding proteins have been identifi ed and have provided important clues as to how intracellular vesicle transportation during endocytosis by Rab5 is regulated. Tu...
متن کاملRab5 affinity chromatography without nonhydrolyzable GTP analogues.
Rab5 is an important small GTPase involved in endocytosis and membrane trafficking. Rab5-binding proteins can be identified using Rab5 affinity chromatography with nonhydrolyzable GTP analogues such as GTPgammaS or GppNHp. However, this method requires significant quantities of the GTP analogue and is thus time-consuming and expensive. In the present report we show a faster and more cost-effect...
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ژورنال
عنوان ژورنال: Methods
سال: 2014
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2014.07.006